Xanthine Oxidase Activity Assay Kit (Colorimetric)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记说明:
- 灵敏度:
- 检测范围:
- 样品类型:
- 样本量:
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CAT.NO. : ARG82198
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Product Details
概述
| 产品描述 | ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) can be used to measure Xanthine Oxidase activity in serum and cell lysate. |
|---|---|
| 反应物种 | Hu, Ms, Rat, All |
| 应用 | FuncSt |
| 靶点名称 | Xanthine Oxidase |
| 偶联标记说明 | Read at 570 nm (colorimetric), or 530/585 nm (fluorometric) |
| 灵敏度 | OD: 0.03 U/L; FL: 0.01 U/L |
| 检测范围 | 0.03 - 25 U/L (colorimetric); 0.01 - 2.5 U/L (fluorometric) |
| 样品类型 | serum and cell lysate |
| 样本量 | 10 µl |
| 別名 | EC 1.17.1.4; XOR; XO; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase; XD; EC 1.17.3.2; Xanthine oxidoreductase |
应用说明
| 应用说明 | Please note that this kit does not include a microplate. |
|---|---|
| 检测时间 | 20 min |
属性
| 形式 | Liquid |
|---|---|
| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | XDH |
| 全名 | xanthine dehydrogenase |
| 背景介绍 | Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014] |
| 生物功能 | Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). [UniProt] |
| 细胞定位 | Cytoplasm. Peroxisome. Secreted. [UniProt] |
| 翻译后修饰 | Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). [UniProt] |
检测图片 (1)
ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 25 U/L Xanthine Oxidase reaction, using ARG82198 Xanthine Oxidase Activity Assay Kit (Colorimetric).
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