XBP1 Rabbit Monoclonal Antibody(ARA844)
Key features and details
- Reactivity:Human, Mouse
- Application:WB, IP
- Host:Rabbit
- Clonality:Monoclonal
- Target:XBP1
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Brand:
CAT.NO. : ARA6667
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Product Details
Background
XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IP |
1:10 - 1:50 |
Overview
|
Immunogen |
Recombinant protein of human XBP1 |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography |
|
Clone Type |
Monoclonal |
|
Product Form |
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA |
|
Gene Name |
XBP1 |
|
Related Names |
TREB5; XBP2; X-box-binding protein 1; XBP-1; Tax-responsive element-binding protein 5 |
|
Gene ID (Human) |
7494 |
|
Gene ID (Mouse) |
22433 |
|
Protein ID (Human) |
P17861 |
|
Protein ID (Mouse) |
O35426 |
Data
Western blot analysis of XBP1 expression in Raw264.7 (A), HL60 (B), U2OS (C) whole cell lysates. (Predicted band size: 29 kD; Observed band size: 29 kD)
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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