P-Glycoprotein Mouse Monoclonal Antibody(ARA851)

Key features and details

Reactivity:Human, Mouse, Rat
Application:IHC
Host:Mouse
Clonality:Monoclonal
Target:P-Glycoprotein

Brand:

CAT.NO. : ARA6676

RMB Please choose

Size:

1mL

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

P-glycoprotein 1 (permeability glycoprotein, abbreviated as P-gp or Pgp) also known as multidrug resistance protein 1 (MDR1) or ATP-binding cassette sub-family B member 1 (ABCB1) or cluster of differentiation 243 (CD243) is an important protein of the cell membrane that pumps many foreign substances out of cells. More formally, it is an ATP-dependent efflux pump with broad substrate specificity. It exists in animals, fungi, and bacteria, and it likely evolved as a defense mechanism against harmful substances. Substrate enters P-gp either from an opening within the inner leaflet of the membrane or from an opening at the cytoplasmic side of the protein. ATP binds at the cytoplasmic side of the protein. Following binding of each, ATP hydrolysis shifts the substrate into a position to be excreted from the cell. Release of the phosphate (from the original ATP molecule) occurs concurrently with substrate excretion. ADP is released, and a new molecule of ATP binds to the secondary ATP-binding site. Hydrolysis and release of ADP and a phosphate molecule resets the protein, so that the process can start again.

Application

Application	Dilution Ratio
IHC-P	1:1000 - 1:5000

Overview

Antibody Type	Recombinant Mouse Monoclonal Antibody
Immunogen	Recombinant protein within human P Glycoprotein aa 935-1,280 / 1,280
Species Reactivity	Human, Mouse, Rat
Molecular Weight	Predicted band size: 142 kDa
Positive Control	Human colon cancer tissue, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue
Conjugation	unconjugated
RRID	AB_3096663
Form	Liquid
Storage Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG1
Purification Method	Protein A affinity purified

Data

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with P-Glycoprotein Mouse Monoclonal Antibody(ARA851) at 1/5,000 dilution.