M13 Major Coat Protein Mouse Monoclonal Antibody(ARA828)
Datasheet
Key features and details
- Reactivity:Species independent
- Application:WB, IP, IF,FCM
- Host:Mouse
- Clonality:Monoclonal
- lsotype:IgG2aκ
- Target:M13 Major Coat Protein
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Brand:
CAT.NO. : ARA6630
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Product Details
Background
Morphogenesis of filamentous phage includes synthesis of the phage major coat protein in precursor form, its insertion into the host cell plasma membrane, its cleavage to the mature form of the protein and its assembly there into virions. At each stage of infection, the major coat protein of coliphage M13 binds to the Escherichia coli cytoplasmic membrane with its antigenic site exposed to the cell exterior, which is at the amino - terminus of the protein. Coat protein synthesized in vitro is initially made with a 23 amino acid amino - terminal “leader peptide”, termed “procoat”, that is also a biosynthetic precursor of coat protein in vivo. The filamentous bacteriophage major coat protein occurs as a membrane - spanning assembly intermediate prior to incorporation into the lipid - free virion. Assembly of coliphage M13 is known to occur as the viral DNA crosses the cytoplasmic membrane, shedding its virus - coded DNA unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein.
Application
M13 Major Coat Protein is recommended for detection of M13 filamentous phage coat protein g8p by Western Blotting (starting dilution 1:200, dilution range 1:100 - 1:1000), immunoprecipitation [1 - 2 µg per 100 - 500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50 - 1:500) and flow cytometry (1 µg per 1 x 10⁶ cells). Molecular Weight of M13 Major Coat Protein: 5 kDa.
Sourse
M13 Major Coat Protein is a mouse monoclonal antibody raised against isolated M13 phage coat proteins.
Data
M13 Major Coat Protein. Western blot analysis of M13 Major Coat Protein expression in M13 phage lysate.
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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