M13 Major Coat Protein Mouse Monoclonal Antibody(ARA828)

Datasheet

M13 Major Coat Protein Mouse Monoclonal Antibody(ARA828)

Key features and details

Reactivity:Species independent
Application:WB, IP, IF,FCM
Host:Mouse
Clonality:Monoclonal
lsotype:IgG2aκ
Target:M13 Major Coat Protein

Brand:

CAT.NO. : ARA6630

US$ Please choose

Size:

200µg

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

Morphogenesis of filamentous phage includes synthesis of the phage major coat protein in precursor form, its insertion into the host cell plasma membrane, its cleavage to the mature form of the protein and its assembly there into virions. At each stage of infection, the major coat protein of coliphage M13 binds to the Escherichia coli cytoplasmic membrane with its antigenic site exposed to the cell exterior, which is at the amino - terminus of the protein. Coat protein synthesized in vitro is initially made with a 23 amino acid amino - terminal “leader peptide”, termed “procoat”, that is also a biosynthetic precursor of coat protein in vivo. The filamentous bacteriophage major coat protein occurs as a membrane - spanning assembly intermediate prior to incorporation into the lipid - free virion. Assembly of coliphage M13 is known to occur as the viral DNA crosses the cytoplasmic membrane, shedding its virus - coded DNA unwinding protein and acquiring from the membrane approximately 2400 copies of the major coat protein.

Application

Application	Dilution Ratio
WB	1:500 - 1:2000
IF/IC	1:50 - 1:200

Overview

Immunogen	Recombinant fusion protein of human MDR3. The exact sequence is proprietary.
Purification Method	The antibody was purified by immunogen affinity chromatography.
Clone Type	Polyclonal
Product Form	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Gene Name	ABCB4
Alternative Names	MDR3; PGY3; Multidrug resistance protein 3; ATP-binding cassette sub-family B member 4; P-glycoprotein 3
Gene ID (Human)	5244;
Gene ID (Mouse)	18670;
Protein ID (Human)	P21439;
Protein ID (Mouse)	P21440;